Plant-parasitic nematodes are pests of agricultural crops and cause crop yield reduce when the roots are damaged. Pineapple is extremely sensitive to root feeding nematodes. Four genera of plant parasitic nematodes were found to be associated with Queen pineapple such as Rotylenchulus reniformis, Pratylenchus brachyurus, Xiphinema sp. and Helicotylenchus sp. as soil samples were collected and isolated.